Western blot analysis was performed on nuclear enriched cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with PMA (50 ng/mL for 2 hours) (Lane 2), HeLa treated with PMA (50 ng/mL for 2 hours) and Trichostatin A (1uM for 1 hour) (Lane 3), U-87 MG (Lane 4) and PC-3 (Lane 5). The blot was probed with Anti-c-Jun Monoclonal Antibody (C.238.2) (Product # MA5-15172, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to c-Fos was observed across the cell lines tested and was enhanced upon treatment.